FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664719
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64350.3865517243450696No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTTCGAAGATT51930.3119445383875597No Hit
GTCCCAGACCCACTGCCACTGAATTTGGCTGGGATACCAGTGGCCCTGGG44620.2680332236251283No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACATTA44510.2673724514467607No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43600.2619060634257193No Hit
CTGGTATCCCAGCCAAATTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT43350.26040430847488377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41150.24718886490753095No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39840.23931966896515267No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAAGTC38890.2336130001519776No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38480.2311501220326073No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38390.23060949025030653No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTTCGAAGATTCTGCAGTTTATTACTGTCATCAATATGATAACTGG38260.22982857767587206No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT34550.20754253420547253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34050.20453902430380144No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33040.19847193430242582No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32640.19606912638108895No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32470.19504793301452075No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAATAACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT32400.19462744162828682No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31890.1915638615285823No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT31100.186818315883942No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29300.17600568023792604No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28610.17186083657361992No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28120.16891739686998225No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27820.1671152909289796No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAATTTGGCTGGGATACCA26210.1574439890455987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25690.15432033874786075No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAATAACTTAGCCTGGTA25330.15215781161865755No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25040.15041577587568833No Hit
GGATACCAGTGGCCCTGGGGGAGGCACCAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGG24760.14873381033075253No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23130.13894236805130475No Hit
ATATGATAACTGGCCTCAGACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT22460.1349176647830655No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22360.13431696280273125No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22350.13425689260469784No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC22060.13251485686172862No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21930.13173394428729412No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21650.13005197874235833No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21240.12758910062298803No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA20770.1247658013154172No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA20590.12368453775081562No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20580.12362446755278218No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20520.12326404636458166No Hit
CTCCCAGGCTCCTCATCTTTGGTGCCTCCCCCAGGGCCACTGGTATCCCA20220.121461940423579No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20180.1212216596314453No Hit
GTTTATTACTGTCATCAATATGATAACTGGCCTCAGACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19910.11959976428454291No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19610.11779765834354027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19540.11737716695730631No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19390.11647611398680496No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19380.11641604378877157No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19270.11575527161040391No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19170.11515456963006969No Hit
GTAATAAACTGCAGAATCTTCGAACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA18910.11359274448120073No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18700.11233127032249887No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC18510.11118993655986385No Hit
GCCCTGGAGTGCCTTGCAATCATTTATTGGAATGATGATAAGCGCTACAG18150.10902740943066067No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18150.10902740943066067No Hit
CCCCAGGGCCACTGGTATCCCAGCCAAATTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG18130.10890726903459383No Hit
GTTATCATATTGATGACAGTAATAAACTGCAGAATCTTCGAACTGCAGGCTGCTGATGGTGAGAGTGAACT17820.10704509289555775No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17580.10560340814275562No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17560.10548326774668879No Hit
CTGCTACTCTGGCTCCCAGACATTACTGGAGAAATAGTGATGACGCAGTC17540.10536312735062194No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17460.10488256576635456No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17380.1044020041820872No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17340.1041617233899535No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17020.102239477052884No Hit
CACCATCAGCAGCCTGCAGTTCGAAGATTCTGCAGTTTATTACTGTCATCAATATGATAACTGGCCTCAGACTTT16830.10109814329024899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4650.041.3428421
GTATCAA26650.038.5158231
TAACGTG451.03345265E-438.131095
GTAGTTT2201.8189894E-1235.52663870
TATCAAC30400.033.8684652
GGTATCA11300.033.7217251
AATGGTA2205.4569682E-1132.56608670
AACGCAG32600.032.109246
ATCAACG32300.032.085823
TGTTAGA10050.031.75598370
CAACGCA33150.031.6781355
TCAACGC32900.031.5006624
AGAGTAC34950.029.26286911
CGCAGAG36300.028.6473088
GCAGAGT36950.028.050489
CTTACGC500.00752687627.75533743
ACGCAGA39250.026.7565177
CAGAGTA40400.025.31527710
TCAATAT30650.024.86284870
AGTACGG34650.024.7611913
Produced by FastQC (version 0.11.5)