FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100046
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27650.040.68241
TCGTATG2850.036.47714240
CTCGTAT2250.035.38770739
TATCAAC32550.034.555022
GGTATCA13250.034.269831
ATCAACG34000.033.2836843
CAACGCA34500.032.9010125
AACGCAG34600.032.8059236
TCAACGC34750.032.5653344
TATACAC4450.031.691173
CGTATGC3450.031.15771341
CGGATAT450.00439118630.99026749
CTTATAC4400.030.4905911
AATCTCG2850.030.31847436
ACGCAGA39300.029.0562937
AGAGTAC39100.028.58785411
CGCAGAG40150.028.5255228
CAGAGTA39450.028.50858710
TTATACA5000.028.2051412
CGATTGT3150.027.00047370
Produced by FastQC (version 0.11.5)