FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100046
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26690.2426262174490885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32500.040.820411
TATCAAC36100.036.549292
CAACGCA38400.034.272225
AACGCAG38850.034.230596
TCAACGC38750.034.049794
ATCAACG39100.033.743463
ACGCAGA42300.031.6026427
GGTATCA14100.031.2006951
GTGGTAT5300.031.127111
CAGAGTA42600.031.0545910
AGAGTAC42800.030.90947511
CGCAGAG43150.030.8194278
GCAGAGT44850.029.6498979
TACCGTA756.330268E-527.4918637
TATATAG658.864634E-426.4308782
AGTACGG37950.026.34850113
CGATTGT2857.4578566E-1125.9618270
GAGTACG38800.025.94722212
GCCTAAT2951.1459633E-1025.08175870
ATAGACG1001.6135422E-524.0520974
Produced by FastQC (version 0.11.5)