FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773661
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA751.8189894E-1145.869262
GTATCAA24300.044.8782581
TAAGACA851.8189894E-1244.62111331
CTAAGAC851.8189894E-1244.61244230
CCCTAAG851.8189894E-1244.58356528
GGTATCA8900.043.292341
ACCCTAA901.8189894E-1242.09852227
TATACAC903.6379788E-1242.0468183
CTTATAC856.91216E-1140.472881
TATCAAC27200.040.093452
CCTAAGA955.456968E-1239.90863829
GACCCTA1009.094947E-1237.87151326
AACGCAG31500.034.8387956
TATTCAC601.3677891E-534.4041719
TCATAAT601.3683948E-534.4019472
CAGAGTA31400.034.28795210
AGAGTAC31550.034.12493511
ATAAGAG1650.033.3594673
GCAGAGT32900.033.351989
CAACGCA33300.032.852315
Produced by FastQC (version 0.11.5)