FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298778
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG150.002387103668.7693253
ACTAGGT252.6101308E-455.0154573
TATCTAA200.007020995552.36945751
CTTATAC651.1716111E-842.324471
ATAAGAG1550.042.148943
TTATACA759.0039975E-1041.261592
GTATAAG1350.040.7568971
ACAAATG350.001241269840.09491357
ACTAGAT702.1871529E-839.43340732
TACTAGA702.1984306E-839.41056431
TATAAGA1400.039.296752
GTATCAA37900.039.0161251
GGTATCA16800.037.2543531
TAAGAGA1500.036.6769684
GTGGTAT5750.036.4818531
TATCAAC41750.035.5788572
GGTTAGG400.002637951634.388631
CTACCCT501.8985444E-434.384664
ATACACA905.3641998E-934.384664
TGGTATC6150.034.105112
Produced by FastQC (version 0.11.5)