FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298778
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16420.12642653324894632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTAA650.001093788541.13661670
CTGGTTA350.00136107139.35054429
GTATCAA38600.038.4741361
GTGGTAT7050.036.5716551
GGTATCA17050.036.091181
TGGTATC7200.034.8454252
TATCAAC42850.034.6488082
TATTGAT400.002645692834.3680882
TAAGGTT400.002647191834.364124
CAGAGTA50050.029.32102810
AGAGTAC50100.029.29176311
AACGCAG50950.029.139246
CAACGCA51800.028.7937775
GCAGAGT51650.028.6112469
GGGTAAG756.315443E-527.5018831
TCAACGC54750.027.3657654
ACGCAGA54600.027.3171717
ATCAACG54800.027.0899623
CGCAGAG55500.026.8741938
CTAAGGA658.8595116E-426.4339374
Produced by FastQC (version 0.11.5)