FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653545
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT99930.3765905609288706No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG80490.30333007354312813No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65460.2466888633884106No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT62970.2373051898498047No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62680.23621231220876224No Hit
GTATGATAATCTCCCTCGGGTCACTTTTGGCCAGGGGACCAAACTGGAAGTCAGACGAACTGTGGCTGCACCAT62360.23500637826002574No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60120.22656484061887022No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC59810.22539659210603175No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTGGCAGCTATTTAAATTGGTATCAGCAGAAA56360.2123951167212163No Hit
AGTCAGGACATTGGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGGA55550.20934259641347705No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55450.20896574205449692No Hit
CTTTTGGCCAGGGGACCAAACTGGAAGTCAGACGAACTGTGGCTGCACCA48950.1844702087207867No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG48770.18379187087462245No Hit
GTGCAGCCACAGTTCGTCTGACTTCCAGTTTGGTCCCCTGGCCAAAAGTG47520.17908119138737047No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT46170.17399365754113835No Hit
GGCCAAAAGTGACCCGAGGGAGATTATCATACTGTTGACAGTAATATGAA45970.17323994882317806No Hit
TTATCATACTGTTGACAGTAATATGAAGCAACATCCTCAGGCAGCAGGCTGTTGATGGTGAAAGTAAAGTCTGTC45200.17033817025903086No Hit
CCCCTAGGCTCCTGATTTACGATGCATCCAGTTTGGCAACAGGGGTCCCG43310.16321562287430588No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43170.16268802677173366No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42770.16118060933581305No Hit
GTCTCCATCCTCCCTCTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTGGCAG42750.161105238464017No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42250.15922096666911623No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42130.15876874143834005No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41940.15805271815627775No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT39040.1471239417458532No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC37980.14312928554066354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36080.13596905272004056No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA35150.1324643071815251No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35130.13238893630972906No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT34330.1293741014378878No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33660.12684917723272074No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA33600.12662306461733266No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33180.125040276309616No Hit
TCCTCAGGCAGCAGGCTGTTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTCCACTGAACCTTGACGGGACC32230.1214601598993045No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32000.12059339487365016No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT31250.11776698718129898No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGACTTCCAGTTTGGTCC31150.11739013282231882No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30830.11618419887358233No Hit
GCCTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCCA29850.1124910261555768No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29720.11200111548890258No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28640.1079310884119169No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28380.10695126707856847No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28100.10589607487342405No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28080.105820704001628No Hit
GCCTGAGGATGTTGCTTCATATTACTGTCAACAGTATGATAATCTCCCTC28050.10570764769393397No Hit
CAGTAATATGAAGCAACATCCTCAGGCAGCAGGCTGTTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTCCA28000.10551922051444389No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC28000.10551922051444389No Hit
GCCACAGTTCGTCTGACTTCCAGTTTGGTCCCCTGGCCAAAAGTGACCCG27920.10521773702725976No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT27840.10491625354007562No Hit
GACTTTACTTTCACCATCAACAGCCTGCTGCCTGAGGATGTTGCTTCATATTACTGTCAACAGTATGATAATCTC27490.10359726328364507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)