FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271705
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44790.35220432411604896No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCAGCTTCAGGGAGA33940.2668857950546707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31600.24848530122945178No Hit
ATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA31110.24463220636861535No Hit
GATCTATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA30330.23849870842687573No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30150.23708328582493582No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCACC28360.2230076943945333No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC26670.20971844885409743No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26510.20846029543015085No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25520.20067547111948134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25420.1998891252295147No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24870.1955642228346983No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24780.19485651153372832No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24470.1924188392748318No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23830.18738622557904547No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23020.18101682387031584No Hit
GTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTG22990.18078092010332586No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCAGCTTCAGGGA21020.16528990607098346No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT20950.16473946394800681No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20880.16418902182503017No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20740.1630881375790769No Hit
GAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCC20470.16096500367616703No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20220.1589991389512505No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC19990.15719054340432725No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19790.15561785162439404No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA19170.15074250710660098No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18730.14728258519074786No Hit
GGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTGCACCAT18390.14460900916486136No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18300.1439012978638914No Hit
GTCTATTACTGTGCGCGGCACCCCTCGTGGTATGGGTGGTTCGACCCCTGGGGCCAGGGAACCCCGGTCACCGT18120.14248587526195147No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18050.14193543313897483No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17900.14075591430402493No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17740.13949776088007831No Hit
GCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGA17670.1389473187571017No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17410.13690281944318847No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17010.13375743588332198No Hit
GTGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGCTAATACC16390.12888209136552897No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15720.1236135739027526No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15360.12078272869887277No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14900.11716553760502632No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14840.11669373007104636No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14730.11582874959208307No Hit
CACCTGCACAGTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCCGGGAAGGGA14660.11527830746910644No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14650.11519967288010978No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14570.11457059616813649No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14400.11323380815519322No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14340.11276200062121325No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14310.11252609685422327No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACT14070.11063886671830339No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13940.10961661706134677No Hit
CTCTGACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTC13910.10938071329435678No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13550.10654986809047697No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGATCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13510.1062353297344903No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13430.10560625302251701No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13350.10497717631054372No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13260.10426946500957376No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13210.10387629206459045No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13080.10285404240763384No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13040.1025395040516472No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC12840.10096681227171396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAA9750.044.00482670
TATACAC2200.037.5089233
GTATCAA20500.035.5586361
GTCGAAT750.002727493234.15300470
CTTATAC2100.032.7471731
TATCAAC23100.031.4062822
ACGAGAC2350.030.72539522
TCGTATG703.7549853E-529.68140640
ACCGATC2350.029.278427
CTATTGA10050.028.89773869
CAACGCA25550.028.6638665
ATCAACG25500.028.5852343
AACGCAG25500.028.5852346
TCAACGC25500.028.5852344
TACACAT2750.027.5065465
ATCTCCG2650.027.2406210
CTCGTAT1155.480433E-827.08003439
TTCGGGG9000.027.03779470
TCAGTAT2550.027.0288532
AGAGTAC26850.026.75750211
Produced by FastQC (version 0.11.5)