FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721040
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1250.055.120711
TTATACA1300.053.0043562
CCTAGGA200.007027549752.35470252
ATAGGAA200.00735127651.76182630
AGCTTAT200.00740189151.67208515
ATACACA1450.049.897214
TATACAC1450.047.521153
TACACAT1550.046.678035
GTATCAA22000.044.31581
GGTATCA8400.043.8833051
AGGGGGG1701.8189894E-1242.2004170
TATCAAC24450.039.737012
GTGGTAT2300.038.9439771
CGAGACT1800.038.2809323
TCCTACA655.5061537E-737.103052
TCGTATG1250.036.08373640
CTCGTAT1003.619789E-1034.6571739
GAGACTC2000.034.45522724
TAAACGC400.002613167334.452834
TGAGTGG951.9152508E-434.3256970
Produced by FastQC (version 0.11.5)