FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018310
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCT200.00699832752.41147254
CTCTTAT200.00743166351.6211851
TATACCT200.007435993851.613585
CTTATAC950.050.715551
TTATACA950.050.7080762
TATACAC1150.047.8734633
TTTAGGC405.0801347E-543.0113143
GTATCAA33900.039.692981
AGGGGGG1403.7216523E-837.11724570
GGTATCA13900.036.8899571
TATCAAC37450.035.925072
GTGGTAT5450.034.0983961
TAAGAGA1950.033.5267684
GTATAAG1750.033.430861
AACGCAG42900.031.6819936
ATTACAT450.00418926631.28958364
TAGTACC553.3075703E-431.2809544
TATAAGA1900.030.7870462
CAGAGTC1900.030.7825110
ACTTTAG450.00467914530.5903281
Produced by FastQC (version 0.11.5)