FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595987
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGTTTCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT48080.3012555866683125No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43940.2753155257530293No Hit
CCATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC41730.2614682951678178No Hit
GTAATACACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTCAAGATACA38620.24198192090537077No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA32750.2052021726994017No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31070.19467577116856216No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29440.184462655397569No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29280.1834601409660605No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25890.16221936644847357No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25080.15714413713896166No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23590.1478082214955385No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23300.14599116408842927No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC22730.14241970642618018No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22390.14028936325922453No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22320.13985076319543957No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21790.13652993414106757No Hit
GTGTATTACTGTGCAAGAGGGGGCACTTTGTTTCGGGGAGTTATTAGCTG21520.13483819103789693No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21440.13433693382214265No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20520.12857247584096862No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT20030.12550227539447376No Hit
GTCTCCATCCTCACTGTCTGCATCCGTAGGAGACAGAGTCACCATCACTT19140.11992578886920759No Hit
GTCTGCATCCGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG19120.11980047456526902No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19060.11942453165345332No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18750.11748215994240555No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18280.11453727379984924No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17820.11165504480926222No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT17680.11077784468169227No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17550.10996330170609159No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT17470.10946204449033732No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT17330.10858484436276736No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC16540.10363492935719402No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16320.1022564720138698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.040.9408531
GTATCAA36900.039.7347151
TCGTATG1700.038.69246740
GTGGTAT5550.037.7403031
TGGTATC5950.035.198732
AGACTCG2750.034.97057725
GTCGTAT806.938353E-834.58897439
TATCAAC43450.033.7405552
ACTCGAC2950.032.60684627
TCAACGC45300.032.363644
CTCGTAT1500.032.28304739
ATCAACG45450.032.2568323
CAACGCA45850.032.1251875
ACGAGAC3100.032.1221722
AACGCAG46750.031.5067376
CGAGACT3250.030.6405723
CTTATAC3200.030.0453031
GACGTCA3100.029.95566431
CGACGTC3150.029.46722830
CGTATGC2300.028.62138741
Produced by FastQC (version 0.11.5)