FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595987
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGTTTCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46830.2934234426721521No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43590.2731225254341044No Hit
CCATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC39690.24868623616608404No Hit
GTAATACACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTCAAGATACA33550.21021474485694433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31650.19830988598278057No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA31600.19799660022293414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26720.16741991006192408No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26510.16610410987056912No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26100.16353516663982853No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26020.16303390942407425No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25130.15745742289880807No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24520.1536353366286818No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24220.15175562206960333No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23590.1478082214955385No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23100.14473802104904362No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22050.13815902009226894No Hit
GTGTATTACTGTGCAAGAGGGGGCACTTTGTTTCGGGGAGTTATTAGCTG21640.13559007686152832No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20210.12663010412992085No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC20000.1253143039385659No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19770.12387318944327241No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT19700.12343458937948742No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19510.12224410349207106No Hit
GTCTGCATCCGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG18590.11647964551089701No Hit
GTCTCCATCCTCACTGTCTGCATCCGTAGGAGACAGAGTCACCATCACTT18190.11397335943212569No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17050.10683044410762745No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16870.10570261537218034No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16840.1055146439162725No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA16440.10300835783750117No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT16050.10056472891069915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39600.039.7494931
GGTATCA16550.037.9198951
GTGGTAT6450.037.7496341
TGGTATC6900.035.7746162
TATCAAC44750.035.165052
ATCAACG47750.033.313683
TCAACGC48100.033.0723084
AACGCAG48850.032.8483626
CAACGCA48850.032.6367725
CGTGGCA11750.031.9550370
CGCAGAG51400.031.0186048
TACGATT450.00474120530.50908527
AGAGTAC51350.030.44786311
CAGAGTA52200.030.21480210
ACGCAGA53850.029.6710327
GCAGAGT54550.029.1017179
GAGTACG42700.025.69454412
AGTACGG42700.025.45365713
TAGACGG700.001383286724.4901125
GTATTAC11250.023.76953
Produced by FastQC (version 0.11.5)