FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182510
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16540.13987196725609086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30500.039.5165941
GGTATCA12450.038.7230871
TATCAAC36050.033.6139952
CAACGCA39300.031.0970025
AACGCAG39500.031.0267036
ATCAACG39550.030.7263473
GTGGTAT4650.030.3628271
TCAACGC40150.030.1814334
AGAGTAC41250.029.12129811
CGCAGAG42000.028.8471348
CAGAGTA41950.028.63536510
GCAGAGT43450.028.1221059
TGGTATC5150.026.7384032
ACGCAGA46300.026.4653877
TTTTACG1759.094947E-1225.59610628
GGATATA951.0660691E-525.3738141
CTCGTAT1801.2732926E-1125.00470439
GTGTATA2350.024.9110681
TCGTATG1851.8189894E-1124.34339340
TACGATT1701.6916601E-1024.33960531
Produced by FastQC (version 0.11.5)