FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182510
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21440.1813092489704104No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17040.14410026130857242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33450.037.3351441
GTGGTAT6900.035.8996931
GGTATCA15300.035.753041
TGGTATC7200.033.4340672
TATCAAC38350.032.461372
ATCAACG40700.030.6715583
CTAGACG450.004695974330.5682893
CAACGCA41750.029.9850855
TCAACGC41650.029.8068334
AACGCAG42100.029.6553636
AGAGTAC42100.028.75671611
CGCAGAG43750.028.2224778
CAGAGTA43550.027.7992610
GCAGAGT44900.027.3464289
TAACGGC3050.026.06961836
ACGCAGA48350.025.964217
TTACGAT1359.5260475E-925.53078730
AATAGCG2900.025.484562
GTGTATA2300.025.428951
TTAACGG3000.025.33883535
Produced by FastQC (version 0.11.5)