FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364607
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35800.2623465950269931No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28320.20753227852414652No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26840.19668666509844956No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24660.1807113696470852No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC22560.1653223235700828No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22160.16239107669827282No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21970.1609987344341631No Hit
GAGTTACAGTTCCCTCGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT21530.15777436287517213No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21090.15454999131618113No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20470.15000655866487567No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19920.14597609421613697No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19710.1444371896084367No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCTCGGGACGTT18970.13901438289558826No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17940.13146642220067756No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17640.12926798704682008No Hit
TCTCTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCACT17520.1283886129852771No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17250.12641002134680535No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACACCTATTTAAATTGGTATCAGCAGAAA16400.12018112174420914No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15760.11549112674931318No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC14320.10493863801079725No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14290.1047187944954115No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14240.10435238863643526No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAC14120.10347301457489227No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13940.10215395348257778No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13750.10076161121846802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6650.042.2680971
GTATCAA50000.041.065521
TATCAAC55750.036.6375472
AACGCAG63250.032.7820246
CAACGCA63500.032.4862865
ATCAACG64150.032.10373
AGAGTAC65150.031.56296211
TCAACGC65750.031.4267044
TATACCG553.3864097E-431.1564676
GCAGAGT67650.030.7511839
CGCAGAG67750.030.6552078
CAGAGTA68300.030.10727510
ACGCAGA71450.029.0677417
GTGTATA1800.028.565331
CGATTGT950.00837397827.15320670
AGTACGG63800.026.26812613
GAGTACG64200.026.26461212
GGTATCA31550.025.4235951
TGTATAA2100.024.4791852
ATAAACG700.001387673224.4764943
Produced by FastQC (version 0.11.5)