FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034579
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41900.040.7257421
GGTATCA16550.039.7042241
CTAATAG650.001537919938.3807970
GTGGTAT5200.038.373021
TATCAAC46350.036.508232
TGGTATC6050.032.4037172
ATCAACG53250.031.971353
TCAACGC53550.031.8564664
CAACGCA53950.031.365275
AACGCAG53950.031.365276
AGAGTAC54350.030.87832311
CAGAGTA54650.030.70881510
GCAGAGT56750.029.9966479
TATACCG1052.4016117E-829.4800765
ACGCAGA57550.029.4601547
CGCAGAG58600.028.9322838
TATACAC1404.8567017E-1027.0234033
ATAGTAC851.4737791E-424.277713
AGTACGG57550.024.26364713
GAGTACG58400.023.9682312
Produced by FastQC (version 0.11.5)