FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969142
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.036.4961241
GTATCAA22250.036.3291931
ACTAACG400.002346554335.2182761
CGCATAT553.2202105E-431.42386237
TATCAAC26300.030.9931492
AACGCAG28300.028.924356
AGAGTAC27750.028.13138611
ATCAACG29450.027.9116633
CAACGCA29350.027.889585
TCAACGC29400.027.8421484
CAGAGTA28050.027.83051710
ACGCAGA30400.027.0380277
GTGGTAT4550.026.4590681
GCAGAGT31000.026.2914759
CGCAGAG31350.026.217348
TGGTATC4650.025.8873862
ACCTAAC1052.3355928E-522.9288253
TTATACA1455.451311E-721.3475282
AGTACGG31350.021.17132413
GAGTACG31600.021.1126612
Produced by FastQC (version 0.11.5)