FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969142
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18470.19058094685814875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4500.041.255791
TGGTATC5000.036.429452
GTATCAA21500.035.818981
CACTAGT400.002640936834.3798261
TATCAAC24300.031.6802442
TCATATA553.3310193E-431.24312
GGTATCA10400.029.7517721
AACGCAG26450.029.2395636
TTAACGG605.386287E-428.7820935
CAACGCA27800.028.0611485
AGAGTAC27000.027.8815411
CAGAGTA27100.027.77865810
TCAACGC28350.027.513914
ATCAACG28650.027.225813
ACGCAGA28500.027.0171537
GCAGAGT29350.026.1175969
CGCAGAG29550.026.0571528
TACACTC1301.9176878E-723.7915925
TAACGGC750.001972157523.04120436
ATACAGT1103.3607263E-521.8735566
Produced by FastQC (version 0.11.5)