FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169454
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGA150.002302230669.3995640
CTTATAC1150.062.7713281
TATACAC1250.057.7496263
TTATACA1300.055.528492
CTACTAG200.00721630652.00700839
TAATCTC1300.050.516635
TAGTAAT1550.046.7301632
GTAATCT1550.042.3414534
GTATCAA44800.041.357151
ATAACAT350.001372135639.2854585
TACACAT1850.039.020015
ATACACA1950.038.7817964
GTAGTAA2000.037.91766431
GGTATCA17300.037.7526441
TATCAAC50350.037.0715072
CTCGTAT1800.036.59752339
GAGTTAC400.002643063434.3747751
TCGTATG2250.032.3864640
AACGCAG59650.031.464596
CAGAGTA59250.030.97153910
Produced by FastQC (version 0.11.5)