FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169454
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25140.2149721151922179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTA150.002160920370.5168962
GTATCAA48000.040.592631
TATAAAC451.0271875E-438.1693082
TATCAAC52200.037.313792
GGTATCA19450.034.982511
GTGTATA1450.033.179161
AACGCAG60750.032.3463366
CAGAGTA59250.032.2402910
GTGGTAT7900.032.1891861
AGAGTAC59400.032.10103211
TGTATAA1500.032.0622182
GCAGAGT61450.031.812879
CAACGCA63150.031.0055685
CGCAGAG63250.030.9618458
ATCAACG63250.030.9552253
ACGCAGA65050.030.2635467
TCAACGC66550.029.3170244
TGGTATC8450.029.2706642
TGATAGG500.00753771627.74646840
CTATGGG851.3248737E-424.66210751
Produced by FastQC (version 0.11.5)