FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620649
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43420.26791735903332553No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38700.23879322419598567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34440.2125074584317764No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGCAACAACTACTTAGCCTGGTACC34360.2120138290277537No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33400.2060902761794812No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32110.19813050203961502No Hit
GTATGGTAGCTCCCCTCCGCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT31760.19597087339701563No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30820.1901707278997488No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATG30660.1891834690917034No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGTT29980.18498761915751036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27700.17091918114286314No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27500.16968510763280636No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27440.16931488557978933No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGCGGAGGGGAGCTACCATACTG26650.16444029521506506No Hit
GTGTATTACTGTGCGAGAGAACACGGTGACCACGTTTTCCCCTACTGGTACTTCGATCTCTGGGGCCGTGGCACC26560.16388496213553952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26400.16289770332749412No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26390.16283599965199125No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGAAGATACA25750.1588869644198096No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25280.15598689167117616No Hit
CTACCATACTGCTGACAGAAATACATTGCAACATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC25270.15592518799567334No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAA24220.14944630206787526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23690.14617600726622482No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23540.14525045213368226No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22890.14123971322599774No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22890.14123971322599774No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT22850.1409928985239864No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT22570.1392651956099069No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22280.13747578902032456No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20730.12791171931738457No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20680.12760320093987038No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20490.12643083110531644No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20280.12513505391975682No Hit
GACACATACTACGCAGGCTCCGTGAAGGGCCGATTCACCATCTCCAGAGACGATTCCAAGAACACGGTGTATCTT19860.12254349954863761No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19760.12192646279360923No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19310.11914979739598149No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC19270.11890298269397012No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA19250.11877957534296446No Hit
CTACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG19150.11816253858793607No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19030.117422094481902No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18860.11637313199835374No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18370.11334965189871465No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18260.11267091146818342No Hit
CATCTATGGTGCATTCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18120.11180706001114368No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGTTGCTAACACTCTGA17240.10637713656689388No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16850.10397069322228318No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATGTTGCAATGTATTTCTGTCAGCAGTATGGTAGCTCC16590.10236639765920937No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16440.10144084252666678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACG200.00805967150.5740053
CTTATAC5100.041.655611
TATACAC5250.041.1014143
TTATACA5350.040.3331642
TCGTATG5450.038.55444740
CGTATGC5400.038.35818541
GTATCAA27900.036.501091
CTCGTAT5400.036.29236639
GGTATCA12900.035.028231
GTATGCC5950.034.3200942
ACGAGAC6200.033.18858322
CACGAGA6250.032.37835321
ATAGCGA552.8619307E-432.06369850
CGAGACA6550.031.93803823
ATACACA6900.031.7614564
GCCGTCT6600.031.23890546
TACACAT7050.031.0856785
TATCAAC33350.030.7336312
CTTGAAA6800.030.68347457
ACACATC6950.030.562716
Produced by FastQC (version 0.11.5)