FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620649
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54200.3344339212253856No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43870.2706940244309533No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37220.22966108022156556No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGCAACAACTACTTAGCCTGGTACC35000.21596286425993538No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34050.21010101508716572No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32160.19843902041712919No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATG30880.19054094995276585No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29940.184740804455499No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29860.1842471750514763No Hit
GTATGGTAGCTCCCCTCCGCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT29200.18017473246828894No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29090.1794959920377577No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGTT28980.1788172516072265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28470.17567036415658172No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27690.1708574774673603No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGCGGAGGGGAGCTACCATACTG27350.16875955250026378No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGAAGATACA26360.16265088862548277No Hit
GTGTATTACTGTGCGAGAGAACACGGTGACCACGTTTTCCCCTACTGGTACTTCGATCTCTGGGGCCGTGGCACC25730.1587635570688039No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25310.1561720026976847No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAA25300.15611029902218185No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24850.1533336336245541No Hit
CTACCATACTGCTGACAGAAATACATTGCAACATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC24190.14926119104136676No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23710.1462994146172305No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23440.14463341537865387No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22960.1416716389545176No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT22840.14093119484848354No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22620.1395737139874211No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22490.13877156620588418No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19700.12155624074059221No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19390.1196434268000042No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19370.11952001944899851No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18930.11680505772687362No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18920.11674335405137079No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA18800.11600290994533671No Hit
CTACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG18310.11297942984569762No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18110.11174535633564084No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17860.11020276444806988No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17750.10952402401753865No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17620.10872187623600175No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17560.10835165418298472No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17180.10600691451387685No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17150.10582180348736832No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17120.10563669246085983No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16840.10390898954678034No Hit
GACACATACTACGCAGGCTCCGTGAAGGGCCGATTCACCATCTCCAGAGACGATTCCAAGAACACGGTGTATCTT16780.1035387674937633No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA16670.10286002706323208No Hit
CATCTATGGTGCATTCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16640.10267491603672355No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16500.10181106457968381No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATGTTGCAATGTATTTCTGTCAGCAGTATGGTAGCTCC16240.10020676901661002No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG200.007129580752.16714544
CGATTGT400.00752783851.4525370
TTATACG200.007678068751.19836835
CAATCGA457.536615E-540.2301659
GGTATCA13150.037.4104961
GTATCAA29600.036.313271
CCTATCG400.002918788733.68675614
TATCAAC34750.030.8270152
ATCAACG35550.030.0366863
TCAACGC35950.029.7961794
CAACGCA36900.028.8482785
AACGCAG37100.028.7853376
AGAGTAC37100.027.87642311
GCAGTAT21150.027.57109370
GCAGAGT38700.027.2470429
TTCGGGG16400.027.1903670
CGCAGAG38800.027.0899898
ATAAGAG1800.026.1991923
CTAATGC659.848777E-425.95934323
TATAAGA1707.2759576E-1225.7604582
Produced by FastQC (version 0.11.5)