FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314088
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGT208.69787E-568.687521
ACTATGT350.005942153354.60506470
TCCTAGC200.00749667551.499242
GGTATCA2900.043.817911
GTATCAA7800.039.6274221
TATACAC557.627248E-637.4539953
TATTCTA700.001990170736.40337870
TATTAGA400.002655987634.332832
TATCAAC9000.033.5698782
TACACAT652.3943825E-531.6918415
CAACGCA10050.030.7458175
CTTGAAA703.3937922E-530.11373957
AACGCAG10150.030.1046496
ATCAACG10050.030.0625763
TCAACGC10150.029.7663944
CAGAGTA9700.029.73152210
CTTATAC703.963049E-529.4375131
TTATACA703.971673E-529.4281392
AGAGTAC9800.029.42813911
GCAGAGT10900.028.0332269
Produced by FastQC (version 0.11.5)