FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079163
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.039.97681
GTATCAA47650.037.840451
GTGGTAT6250.036.677811
CTTATAC2500.035.582951
TATACAC2700.034.2112053
TCGTATG2550.033.99405340
CTCGTAT2550.032.5929639
TGGTATC6950.032.488342
TTATACA2850.032.4106142
AGACTAG2700.031.71672225
GAGACTA2700.031.70348424
TATCAAC57250.031.6117522
CTAGGCA3000.029.72132328
AACGCAG62450.029.1438926
ATCAACG62700.029.0276873
TCAACGC63050.028.9208134
CAACGCA63300.028.7525445
ACTAGGC3100.027.64989727
CTAGATC756.5262575E-527.3689614
CTAGACA756.5262575E-527.3689614
Produced by FastQC (version 0.11.5)