FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297593
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28790.2218723436393384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTC150.002343064969.09299536
GTGGTAT6300.042.5146561
GTATCAA44600.041.4990841
TGGTATC6800.039.3809932
TATCAAC48300.038.3126832
GGTATCA18000.037.2003251
AGAGTAA400.002657793734.33611711
TTACCTC400.002660306234.32954
CAGAGTA53700.033.56882510
AACGCAG55900.033.5388956
AGAGTAC53600.033.37521711
GCAGAGT55700.033.1648649
CAACGCA57950.032.0487635
CATTACA553.3517517E-431.2110422
ACGCAGA60050.031.049517
GTATAAG1900.030.7241551
TATAAGA1900.030.7182352
GTTAACG450.004700701730.5622226
ATCAACG61400.030.247983
CGCAGAG60850.030.2451218
Produced by FastQC (version 0.11.5)