FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128519
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35760.1680041380885019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.040.1621441
GTATCAA60850.040.0522541
TATCAAC68650.035.4915162
ATCAACG74850.032.50273
CAACGCA75100.032.351795
AACGCAG76450.031.8284456
TCAACGC76650.031.739434
AGAGTAC78950.030.08265711
CGCAGAG81450.029.7922928
ACGCAGA81700.029.7011247
CAGAGTA80400.029.66837110
GCAGAGT86850.028.0190689
GTGGTAT9700.027.9960441
AGTACGG67400.024.88513613
GAGTACG68750.024.44647212
TTAACGG3400.024.39014835
TGGTATC11350.022.1027282
GTACGGG72700.021.98152414
TACGGGG29650.021.67688815
GTATAAG7950.021.6194271
Produced by FastQC (version 0.11.5)