FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845074
Sequences flagged as poor quality0
Sequence length20-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAATAA200.006795445552.80200662
TAACGTT200.0074241951.63355611
TCGTATG1700.044.87317340
GGTATCA9650.043.166841
TTATACA1800.042.0792582
GTATCAA26550.041.4932671
CTTATAC1850.040.9395561
GTGGTAT3250.040.2524571
TATACAC1900.039.864563
CCTAGTG350.001287430439.7966648
TTATACC350.001361432339.3468364
TGGTATC3400.038.4788932
TATCAAC29600.037.1036642
CTCGTAT1900.036.48883439
CTTGAAA1950.035.92352357
TACACAT2300.034.4284865
AACGCAG33400.032.8822946
AGAGTAC33100.032.03048711
CAGAGTA33250.031.88598410
ATACACA2600.031.7801384
Produced by FastQC (version 0.11.5)