FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908584
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTAC150.002367525868.9106122
GCATTTA200.006978825752.4480156
ATTCTAG200.007254109351.93739738
CGACTAA200.00739635151.68295718
GACTAAT200.00739635151.68295719
GACTAGC709.094947E-1249.2462626
AGACTAG709.094947E-1249.23541325
CTTATAC709.094947E-1249.224571
TTATACA709.094947E-1249.2164462
GTATCAA27000.041.0934031
GGTATCA10750.041.0281071
AGGGGGG806.9227004E-540.80718270
GTGGTAT3850.040.274651
AGTTTAG350.001325010739.56478537
GTATAAG1050.039.3796581
GTACAGT350.001356651439.3753246
TGGTATC3950.039.2485582
ATAAGAG1150.038.9473273
TATAAGA1100.037.5834662
TATCAAC30050.037.0310752
Produced by FastQC (version 0.11.5)