FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414179
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8600.2076396920172196No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5320.12844687924786144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4510.10889011755786748No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4270.10309552150157299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7250.037.2696651
GTGGTAT1350.035.4699631
GTACTGT501.9562065E-434.203181
GGTATCA3500.034.2031751
TATCAAC8500.031.7811572
CGTACCG652.4610144E-531.56835212
ATCAACG8850.030.9106583
CAACGCA9100.030.0614665
AACGCAG9050.029.8533126
TCAACGC9200.029.3630284
GAATAAT900.00589671629.16656370
ATATTAT900.00589671629.16656370
CCTCGTA500.00806688727.35923812
AGAGTAC9750.027.35923811
TAGAACT659.108E-426.3037824
CGCAGAG10150.026.281048
ACGCAGA10250.026.0246417
GCAGAGT10300.025.8983069
TAATGAG851.3464349E-424.59788947
CAGAGTA10950.024.36096410
Produced by FastQC (version 0.11.5)