FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697104
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCCCGAGTCTCAGGCTGTTCATTTGCAAATACA23940.34342078083040695No Hit
GTGTATTACTGTGCGAAGCATAACAGTCTGTCGACGACTTTGCTCGACGC21420.307271224953522No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15760.22607817484908993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15620.22406986618926303No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15430.22134430443664074No Hit
GTCATACACTGGGTCCGCCAGGCTCCAGGCGAGGGGCTGAAATGGGTGAC14910.21388487227156924No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13690.196383896807363No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13540.19423213752897703No Hit
CTCCTGTGTTGCGTCTGGATTCTCCTTCACTCAACATGTCATACACTGGGTCCGCCAGGCTCCAGGCGAGGGG13310.19093277330211847No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13300.19078932268355941No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13000.1864858041267874No Hit
GTGTATGACATGTTGAGTGAAGGAGAATCCAGACGCAACACAGGAGAGTC11720.16812412495122678No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11710.16798067433266772No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11090.15908673598200557No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10520.15091005072413874No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10480.15033624824990244No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10290.14761068649728018No Hit
GATCAGGAGTTTGGGAGCCTTTCCTGCCTTCTGCTGATACCAGGCTAAGT10030.14388097041474443No Hit
TCTTCAGACTGCAGGCTGCTGATGGTGAGGGTGAAATTTGTCCCAGATCCACTGCCACTGAACCGGGATGGGACC10020.14373751979618535No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9750.13986435309509054No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9680.13886019876517708No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9430.1352739333012004No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9370.13441322958984597No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG9080.13025316165163303No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACAAGATGTGACAT9030.1295359085588377No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8880.1273841492804517No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8800.12623654433197914No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8780.12594964309486104No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8610.12351098257935689No Hit
GTGCAGCCACAGTTCGCTTGAGATCCACTTTGGTCCCAGGGCCGAAAGCT8570.12293718010512061No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGTGT8550.12265027886800246No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT8480.12164612453808901No Hit
CAGTAATACACAGCCGTGTCCCCGAGTCTCAGGCTGTTCATTTGCAAATA8460.12135922330097086No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8430.12092887144529367No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8420.1207854208267346No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8390.12035506897105741No Hit
GTGGAGGGCCGATTCACCGTCTCAAGAGACAATTCGAAAAACACAGTGTA8060.11562119855860818No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG8000.11476049484725379No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7990.1146170442286947No Hit
CTGTTATGCTTCGCACAGTAATACACAGCCGTGTCCCCGAGTCTCAGGCT7870.11289563680598591No Hit
GTATTACTGTGCGAAGCATAACAGTCTGTCGACGACTTTGCTCGACGCCTGGGGCCAGGGAACTCTGGTCCTCGT7820.11217838371319058No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7820.11217838371319058No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7770.11146113062039523No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7630.1094528219605683No Hit
GCCTGAGACTCGGGGACACGGCTGTGTATTACTGTGCGAAGCATAACAGTCTGTCGACGACTTTGCTCGACGC7560.10844866763065483No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7380.10586655649659162No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7350.10543620464091442No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC7330.10514930340379627No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7310.10486240216667814No Hit
CTCCTGATCTATGCCGCATCCACTCTGCAATCAGGGGTCCCATCCCGGTT7290.10457550092956001No Hit
GCACTGAACACAGAGGACTCACCATGGAATTTGGGCTGAGCTGGGTTTTC7240.10385824783676466No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7240.10385824783676466No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7230.1037147972182056No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7160.10271064288829214No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7090.10170648855837866No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7090.10170648855837866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG252.5894176E-455.100672
TACCCGT200.007316276451.82415436
TAACGAG200.007330860551.7980634
CTAATTA200.00741040951.6568715
GTGGTAT1750.041.331431
TAACACG350.001359436339.357624
GTATCAA10050.037.698651
TCTTGAG8400.036.9837270
GAGTTTA1550.033.33181
TATCAAC11850.031.9676882
ACCCTTA450.004361136431.0321955
ATGTAAC450.00465908230.6158751
ATCAACG13150.028.8073833
TCAACGC13200.028.6982634
CAACGCA13200.028.4373685
AACGCAG13500.027.805436
CTCTTGA8200.027.76253369
AGAGTAC13550.026.9384311
CAGAGTA13650.026.7410810
ACGCAGA14150.026.5262457
Produced by FastQC (version 0.11.5)