FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697104
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTGCGAAGCATAACAGTCTGTCGACGACTTTGCTCGACGC20440.2932130643347334No Hit
GTAATACACAGCCGTGTCCCCGAGTCTCAGGCTGTTCATTTGCAAATACA20350.2919220087677018No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16080.2306685946429801No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14550.2087206500034428No Hit
GTCATACACTGGGTCCGCCAGGCTCCAGGCGAGGGGCTGAAATGGGTGAC14250.20441713144667079No Hit
CTCCTGTGTTGCGTCTGGATTCTCCTTCACTCAACATGTCATACACTGGGTCCGCCAGGCTCCAGGCGAGGGG13660.19595354495168582No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13660.19595354495168582No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13010.18662925474534645No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12080.1732883472193532No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11580.16611581629139985No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11580.16611581629139985No Hit
GTGTATGACATGTTGAGTGAAGGAGAATCCAGACGCAACACAGGAGAGTC11080.15894328536344648No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11050.1585129335077693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10690.15334871123964286No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10550.15134040257981593No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10250.1470368840230439No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9680.13886019876517708No Hit
TCTTCAGACTGCAGGCTGCTGATGGTGAGGGTGAAATTTGTCCCAGATCCACTGCCACTGAACCGGGATGGGACC9510.13642153824967293No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9450.13556083453831852No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9380.13455668020840506No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9240.13254837154857813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9080.13025316165163303No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8970.1286752048474833No Hit
GATCAGGAGTTTGGGAGCCTTTCCTGCCTTCTGCTGATACCAGGCTAAGT8830.12666689618765636No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8800.12623654433197914No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8780.12594964309486104No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8750.12551929123918384No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACAAGATGTGACAT8670.1243716862907113No Hit
GTGCAGCCACAGTTCGCTTGAGATCCACTTTGGTCCCAGGGCCGAAAGCT8560.12279372948656156No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG8510.1220764763937662No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8490.12178957515664808No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8480.12164612453808901No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG8220.11791640845555326No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8110.11633845165140352No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT8070.11576464917716725No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8010.11490394546581285No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG7990.1146170442286947No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7940.11389979113589938No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7880.11303908742454497No Hit
CTGTTATGCTTCGCACAGTAATACACAGCCGTGTCCCCGAGTCTCAGGCT7740.11103077876471804No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGTGT7730.11088732814615897No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC7650.10973972319768643No Hit
GCCTGAGACTCGGGGACACGGCTGTGTATTACTGTGCGAAGCATAACAGTCTGTCGACGACTTTGCTCGACGC7550.10830521701209576No Hit
GTATTACTGTGCGAAGCATAACAGTCTGTCGACGACTTTGCTCGACGCCTGGGGCCAGGGAACTCTGGTCCTCGT7500.10758796391930042No Hit
CTCCTGATCTATGCCGCATCCACTCTGCAATCAGGGGTCCCATCCCGGTT7490.10744451330074135No Hit
GTGGAGGGCCGATTCACCGTCTCAAGAGACAATTCGAAAAACACAGTGTA7450.1068707108265051No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7300.10471895154811907No Hit
GTCTCCATCCTCCCTGTCTGCATCAGTAGGAGACAGAGTCACCATCACTT7140.10242374165117399No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7090.10170648855837866No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7070.10141958732126054No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7000.10041543299134706No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7000.10041543299134706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAG8700.041.12119770
GTATCAA12150.038.2210271
GTGGTAT1750.033.4160961
TACGGGT1253.6379788E-1233.0182315
TATCAAC14400.032.0055162
CATTTTG7600.030.81138470
ATCAACG15100.030.7495963
TATGGGA450.00469155230.5724354
TCAACGC15250.030.4471384
TGTTAGA3650.030.29551570
AACGCAG15350.030.2509566
CAACGCA15500.029.9560555
AGAGTAC15850.029.0817411
CAGAGTA16100.029.05747610
ACGCAGA16050.028.9336767
CTCTTGA8350.028.87313369
CGCAGAG16550.028.2673918
ATTGCTC1254.2455213E-927.53889526
GTCAATT658.7443483E-426.49111628
ACCGTGT658.8031904E-426.460718
Produced by FastQC (version 0.11.5)