FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686930
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63340.37547497525089957No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48140.28537046587588105No Hit
ATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA42920.2544266804194602No Hit
GATCTATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA41670.2470167701090146No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39730.235516589307203No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38840.23024073316616575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38670.22923298536394518No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38230.22662469693466833No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37900.22466848061271066No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34740.20593622734790418No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33640.19941550627471205No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCACC33200.1968072178454352No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33090.19615514573811602No Hit
GTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTG32430.1922427130942007No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32300.19147208242191435No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCAGCTTCAGGGAGA30690.18192811794206043No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27560.16337370252470462No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27210.16129892763777987No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26910.15952054916327293No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA26820.15898703562092084No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT26720.1583942427960852No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26700.15827568423111807No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26560.15744577427634815No Hit
GAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCC26180.1551931615419727No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC25970.15394829660981782No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC25830.15311838665504793No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25560.15151784602799168No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24490.14517496280225026No Hit
GGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTGCACCAT23800.14108469231088427No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23170.13735009751441968No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23120.13705370110200188No Hit
GCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGA22710.1346232505201757No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22480.13325982702305372No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21820.12934739437913842No Hit
GTGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGCTAATACC21540.1276875744695986No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21050.12478288962790393No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20640.12235243904607777No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20550.12181892550372571No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20360.12069261913653798No Hit
GTCTATTACTGTGCGCGGCACCCCTCGTGGTATGGGTGGTTCGACCCCTGGGGCCAGGGAACCCCGGTCACCGT20330.12051478128908728No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA20320.12045550200660371No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20150.11944775420438311No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18930.1122156817413882No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18730.1110300960917169No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18560.1100223482894963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18500.10966667259459492No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18120.10741405986021946No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17730.10510216784336043No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17650.10462793358349191No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17550.10403514075865625No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17250.1022567622841493No Hit
CTCTGACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTC17230.10213820371918218No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACT17210.10201964515421506No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17000.10077478022206018No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCAGCTTCAGGGA16880.10006342883225741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAA9350.044.35328770
GTATCAA30300.042.046681
TATCAAC37650.034.1117172
TCAACGC38700.033.1862074
ATCAACG38600.033.182983
CAACGCA38900.033.0155835
AACGCAG38800.033.0119366
CTATTGA9000.030.71518969
TAAGGCG1800.030.63601929
AGAGTAC41450.029.98320611
CGCAGAG42600.029.9020128
CAGAGTA42500.029.32344610
TGGATTG11000.029.00022570
GCAGAGT44050.028.838719
GACTAAG2200.028.18066426
TATACAC2800.025.823853
GAGCAAA22750.025.52019970
GAGTACT14100.025.14858212
CTCGTAT1251.2285454E-724.91572439
ACGAGAC2500.024.79163222
Produced by FastQC (version 0.11.5)