FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732357
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA39270.5362138956820239No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33220.45360391175342085No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32080.4380377329635683No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGT26580.3629377475739291No Hit
GTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCAAGACTATGC25290.3453233873643592No Hit
GTGTGGAGACTGTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATC25180.3438213876565664No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24560.33535557112173436No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23260.31760466548418326No Hit
AGCCAGGACAGTCTCCACACCTCCTAATCTATTTGGGCTCTTATCGGGCC21690.2961670332911408No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21030.2871550350443841No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19780.2700868565467388No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19540.26680976627519093No Hit
ATCCAAGACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC19150.26148449458392564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18990.259299767736227No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTC18210.24864922435369635No Hit
GTCTCCACACCTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCC18120.24742031550186588No Hit
GGCACAGACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTA17790.24291431637848754No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17180.23458504527163665No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17110.23362922727576851No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17000.23212722756797574No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCC16480.22502686531295527No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16220.22147668418544508No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15800.21574177621023627No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15420.2105530499469521No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15380.21000686823502746No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15210.2076855959593477No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14980.20454505111578095No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14810.2022237788401012No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14720.20099486998827074No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14710.20085832456028957No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAA14440.19717159800479822No Hit
GTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACACCTCCTAATCTATTTGGGCT14310.19539650744104312No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGTTCTCCAGGGGTGACGGGCAGGGAGACTGGAGA14180.19362141687728798No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTC14050.19184632631353288No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13000.17750905637551084No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAGGTGTGGAGACTGTCCTGGCTTCTGCAGGTA12680.17313960268011366No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA12670.1730030572521325No Hit
CCCCTGGAGAACCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG12580.17177414840030206No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12250.16726814927692368No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11820.1613966958737337No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAAAATCAGCAGAG11810.16126015044575254No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC11710.15989469616594093No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11580.15811960560218583No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11480.1567541513223742No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC11470.15661760589439302No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT11370.15525215161458142No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCC11350.15497906075861909No Hit
CTCCTGCATAGTCTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACACCTCCTAAT11310.15443287904669445No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11200.15293087933890168No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGT11160.152384697626977No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10890.14869797107148564No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10840.1480152439315798No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA10740.1466497896517682No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10650.14542088079993773No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10310.14077833624857822No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10270.14023215453665358No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAACCGGCCTCCATCTCCT10100.1379108822609738No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9970.1361357916972187No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGA9950.1358627008412564No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAAC9710.13258561056970847No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA9570.13067397457797222No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9560.13053742914999106No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA9500.12971815658210406No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA9440.12889888401421712No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGTTCTCCAGGGGTGACGGGCAGG9410.12848924773027362No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9230.1260314300266127No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9120.12452943031881993No Hit
GATTAGGAGGTGTGGAGACTGTCCTGGCTTCTGCAGGTACCAATCCAAAT8990.12275433975506482No Hit
GTGTGAAGTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG8910.12166197633121552No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA8670.11838488605966763No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8630.11783870434774298No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8510.11620015921196902No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8420.11497125036013857No Hit
ACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGC8340.11387888693628927No Hit
GACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACT8280.1130596143684023No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8170.11155761466060951No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTC8130.11101143294868486No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCT8080.11032870580877906No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8030.10964597866887323No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7980.10896325152896744No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7860.10732470639319348No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT7720.1054130704014572No Hit
CTTATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGC7470.10199943470192815No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7460.101862889273947No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7390.10090707127807885No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7360.10049743499413537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)