FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619262
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGTTTAGGTTCAGGGAGA17310.2795262748239033No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15080.2435156686507488No Hit
GTCTATTACTGTGTGACACATTCTCCCGGTGGCTACCCGCCCTACTACTC14990.24206232580071116No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT14150.22849779253369334No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11460.18505898957145764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10870.17553151977676654No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10820.17472410708230118No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10300.16632701505986158No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9860.15922178334856651No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9520.15373137702620215No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9440.1524395167150576No Hit
ACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTCGACACGTCC9160.14791800562605165No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8480.13693719298132292No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8410.13580681520907145No Hit
GAGTAGTAGGGCGGGTAGCCACCGGGAGAATGTGTCACACAGTAATAGAC7810.12611786287548726No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG7680.12401858986987738No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7490.12095042163090905No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7390.11933559624197836No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7280.11755928831415459No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7200.11626742800301003No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7130.11513705023075856No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7060.11400667245850707No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTCGACACGTCCAAGAACCAGTTCTCCCTGAAC7050.11384518991961398No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7000.11303777722514864No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA6860.11077702168064568No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6850.1106155391417526No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6830.11029257406396646No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA6660.1075473709027843No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6520.10528661535828131No Hit
GTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGA6390.10318734235267141No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6350.10254141219709914No Hit
CTACTACTCTGACTACTGGGGCCAGGGAATTCTGGTCACCGTCTCCTCGGCCTCCACCAAGGGCCCATCGGTCTT6280.10141103442484764No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6250.10092658680816843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG300.00750931651.47950469
TTACACA200.00997905247.908984
CTTATAC4200.044.8707661
TTATACA4300.044.5664942
TATACAC4250.044.3392943
TTGAAAA3900.043.38834458
CTTGAAA3750.042.65590357
GATCCGA309.224099E-442.58576211
AAGGGGG2850.041.5448669
CGTATGC3950.040.8573341
TACACAT4750.040.3444065
GAAAAAA5300.040.2204960
TGAAAAA5150.040.1698759
TGCTTGA3750.039.83261555
TCGTATG4100.039.07087340
GTCGTAT350.001474373338.71196439
CTAGCGC4850.038.49072328
CTCGTAT3700.038.45039439
ACTAGCG4850.038.42506827
TAGCGCT4900.038.194329
Produced by FastQC (version 0.11.5)