FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619262
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41220.6656310253172325No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14710.23754081471170524No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGTTTAGGTTCAGGGAGA14600.23576450678388147No Hit
GTCTATTACTGTGTGACACATTCTCCCGGTGGCTACCCGCCCTACTACTC14450.2333422687004854No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11150.18005303086577248No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10360.16729591029322No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA9920.16019067858192496No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9860.15922178334856651No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9540.1540543421039883No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9430.15227803417616453No Hit
ACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTCGACACGTCC9080.14662614531490709No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9010.1454957675426556No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8360.1349994025146061No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8170.13193123427563777No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8040.12983196127002788No Hit
GAGTAGTAGGGCGGGTAGCCACCGGGAGAATGTGTCACACAGTAATAGAC7880.12724824064773876No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7750.12514896764212885No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTCGACACGTCCAAGAACCAGTTCTCCCTGAAC7740.12498748510323579No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7680.12401858986987738No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7510.12127338670869518No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7480.12078893909201598No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA7350.11868966608640608No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7170.11578298038633084No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG7140.11529853276965163No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6890.11126146929732487No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6880.1110999867584318No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT6830.11029257406396646No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6780.10948516136950112No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6690.10803181851946349No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6630.10706292328610507No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6480.10464068520270903No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA6430.10383327250824369No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6390.10318734235267141No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6350.10254141219709914No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6280.10141103442484764No Hit
CTACTACTCTGACTACTGGGGCCAGGGAATTCTGGTCACCGTCTCCTCGGCCTCCACCAAGGGCCCATCGGTCTT6240.10076510426927536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACATT200.0099977147.8863334
CGACGGA5600.039.95216469
GTAGTAT400.001770251337.2966252
GGTATCA4600.032.63141
GTATCAA9600.032.2697941
GTATTAG502.9271023E-431.9371171
GTTAGCC400.003795999331.9242213
GACCGGA1050.00420522131.25576670
CGATTAA553.3539516E-431.20390141
ACGATTA553.5052464E-430.96999240
TCGACGG6750.030.10866268
TATAAGA555.126342E-429.0243642
TGTCGAC6600.028.7163866
ACGTGTC6250.028.54680363
GTCGACG6800.028.47357667
ATTCTGG8150.028.18771470
GACGTGT6500.027.16096362
TATCAAC11300.027.1236552
GTGTCGA6750.027.07143865
AATGCCA7300.026.97415470
Produced by FastQC (version 0.11.5)