FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934034
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACTA150.002376637468.844045
TATAAAA200.007122919452.17777345
CGTATAA200.007148861452.12982643
ATTTAGT200.00719020852.05385241
TACTAGG200.00733866151.7855232
CTTATAC1000.051.6468541
CTTTAGT200.007421589551.6385615
TATGCTC200.00742473251.633035
TTATACA1050.049.1743132
TATACAC1150.047.891513
CATAACT451.890252E-645.8960274
GTATCAA29300.044.8898741
TCGTATG1100.040.9855840
GCCATAT806.99316E-540.73743470
TATCAAC32450.040.733612
GGTATCA10400.040.7215581
TGTTTTA350.001362045239.34366615
TATAGGC350.001362762439.3394552
TGTAGCA350.001362762439.3394552
AAGTCTA350.001362762439.3394555
Produced by FastQC (version 0.11.5)