FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934034
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20040.21455321754882584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT100.009454825131.6869570
CAGTAAT200.00742308651.6359251
CCTAATA200.007429375351.6248662
GTATCAA32900.042.6898881
GTGGTAT4400.041.4652141
GGTATCA12350.040.4167861
CTCATAA350.001317782439.6089440
GTTAAAC350.001362387139.3416561
TGGTATC4650.039.2274972
TATCAAC35900.039.2099722
AATTTTG400.002620198334.43501722
AACGCAG41200.034.4147346
CAGAGTA40750.033.6968810
AGAGTAC40850.033.61439511
CAACGCA42600.033.1993945
GCAGAGT43400.032.590919
ATCAACG43900.032.057783
TCAACGC45750.031.0623024
ACGCAGA45850.030.9244737
CGCAGAG46450.030.5250198
Produced by FastQC (version 0.11.5)