FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320568
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39940.3024456143114175No Hit
GTCCCCACACCTCCTGATCTTTTTGGGTTCTAATCGGGCCTCCGGGGTCC29310.22194994880990604No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28640.2168763744085878No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28090.21271149990004298No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23750.17984685377807125No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23220.17583342925165535No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23120.17507617934101083No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21990.16651925535072787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21920.1659891804132767No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG19740.1494811323612264No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19700.1491782323969686No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19690.1491025074059041No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19260.14584633279013273No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19200.14539198284374602No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA18950.14349885806713475No Hit
AGCCAGGGCAGTCCCCACACCTCCTGATCTTTTTGGGTTCTAATCGGGCC18440.13963688352284773No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17980.136153533933883No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG17110.12956545971127575No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA16720.12661218505976216No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT16590.1256277601759243No Hit
GTGTGGGGACTGCCCTGGCTTCTGCAGGTACCAATTAAAATAGTTGTATC15660.11858533600693036No Hit
CTACAATCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCG15430.11684366121244796No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14330.10851391219535837No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA14230.10775666228471385No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13800.10450048766894246No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG13760.10419758770468465No Hit
TTATTACTGCATGCAAGCTCTACAATCTCCGTACACTTTTGGCCAGGGGA13700.10374323775829794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG4250.049.916223
CGTATGC4300.045.91187741
TATCTCG4450.044.0954536
GACGGAC4900.043.32748826
TCGTATG4700.042.692340
ACGAGAC5200.042.11451722
CCGTCTT4700.041.54955347
CTCGTAT4600.041.30404739
TATGCCG5000.040.98119443
AGACGGA5300.040.04534525
GTATCAA30700.038.5503581
ACGGACT5550.038.264727
GGTATCA13950.038.250851
CACGAGA5650.038.15030321
CTTATAC5650.038.140191
CGAGCCC5700.037.80419515
CCCACGA5800.037.74497219
ATGCCGT5450.037.63649444
ATCTCGT5350.037.3551637
TAGACCG557.8418125E-637.3160555
Produced by FastQC (version 0.11.5)