FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320568
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39800.3013854644365152No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39160.29653906500839033No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28520.21596767451581442No Hit
GTCCCCACACCTCCTGATCTTTTTGGGTTCTAATCGGGCCTCCGGGGTCC28480.2156647745515566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24360.18446607823300276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23380.17704502910868655No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22650.17151710476098164No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22390.1695482549933059No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22270.1686395551005325No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21370.1618243059047319No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG19990.15137425713783767No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19510.147739457566744No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA18420.13948543354071885No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18400.13933398355858995No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17770.13456330912152953No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17520.13267018434491826No Hit
GTGTGGGGACTGCCCTGGCTTCTGCAGGTACCAATTAAAATAGTTGTATC16930.12820240987211565No Hit
AGCCAGGGCAGTCCCCACACCTCCTGATCTTTTTGGGTTCTAATCGGGCC16800.12721798498827777No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16780.12706653500614887No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA15830.11987266085502601No Hit
CTACAATCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCG14880.11267878670390317No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14710.11139146185580749No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14320.1084381872042939No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13970.10578781251703812No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG13700.10374323775829794No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13680.10359178777616905No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13560.10268308788339561No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG13450.10185011298168667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)