FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914118
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8900.039.841021
TGGTATC3500.037.3744742
GTATCAA18200.035.749811
GTGGTAT3800.034.425741
TATCAAC21850.029.6186942
CAACGCA22600.028.7880925
TCAACGC23000.028.137764
AACGCAG23250.028.1313256
ATCAACG23250.027.8352053
TAATACC500.00781794127.5390834
CAGAGTA23200.027.30018410
ACGCAGA24250.026.971277
ACTAGGT658.7681273E-426.4798893
AGAGTAC23900.026.35657511
CGCAGAG24900.026.2671998
TTATACA1501.0695658E-925.244162
GCAGAGT25950.024.9376899
TATCTAG1001.5895082E-524.0980171
TACTAGG953.1110784E-421.7413832
TAGGTCG800.002936386521.5149085
Produced by FastQC (version 0.11.5)