FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914118
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13830.15129337787900468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8900.035.577251
GTATCAA18250.034.8885881
TGGTATC3450.029.918112
GTGGTAT3700.028.8359681
TAAACGT605.496415E-428.68250520
TATCAAC22000.028.619392
ATCAACG23300.027.3164353
CAACGCA23500.026.9360855
AGAGTAC23300.026.87640811
TCAACGC24000.026.804944
CAGAGTA23650.026.47865910
GTAAACG658.7809504E-426.4732619
AACGCAG24000.026.3763566
ACGCAGA24900.025.4257767
GCAGAGT25750.024.7201029
CGCAGAG26050.024.3033338
ATTCTAA1151.7328985E-623.9423471
ACGGGTA1253.584255E-622.02093516
GTGTTAA1103.327153E-521.9018061
CTGTATA953.12096E-421.7311869
Produced by FastQC (version 0.11.5)