FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093237
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21610.19766985566716092No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15030.13748162566762742No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14490.13254216606280247No Hit
GTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT14080.12879183562210206No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12210.11168666995354164No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12070.1104060693152537No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11980.10958282604778286No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11300.10336276580466999No Hit
CTACAAACTCCTCGGACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACG11110.1016248077955649No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT11010.10071009305393068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26350.040.5853271
TATCAAC28800.037.0028152
GGTATCA10250.036.3486181
TCAACGC29950.035.4700974
ATCAACG30100.035.2917253
AACGCAG30750.034.435136
CAACGCA30900.034.2679755
ACGCAGA34600.030.6006777
AGAGTAC34900.030.03842711
CGCAGAG35800.029.5749598
TAATACG703.8575374E-529.5639024
CAGAGTA35700.029.3666410
GTGGTAT4200.027.1052021
AGTACGG28750.026.50961113
GAGTACG29150.026.14464612
TAGTACG809.5779724E-525.8684144
TGGTATC4450.024.8004042
ACGATTT2950.023.42137532
TGTGCGA3400.023.32909210
GCGAATA750.001855229323.2849457
Produced by FastQC (version 0.11.5)