FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846595
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12290.14516976830715986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAT350.005225984456.42164270
ACTAACT200.00726415351.9189237
ATTAATA200.00736689251.73484419
TATACAA200.00737205751.7256746
GTATCAA24350.041.064131
GGTATCA9750.039.2538221
TGGTATC3550.038.8434942
GTGGTAT3500.038.4202421
TATCAAC27150.036.8226282
CAGAGTA30300.031.75239610
AGAGTAC30300.031.75051911
AACGCAG31650.031.705476
GCAGAGT32450.030.711299
CAACGCA32500.030.6586065
ATCAACG34100.029.4205553
CGCAGAG34250.029.1979478
ACGCAGA34400.029.0706337
TCAACGC36750.027.2052634
CCACACT658.690983E-426.5197184
TGTATAA658.693981E-426.5181522
Produced by FastQC (version 0.11.5)