FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698314
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11750.1682624149021787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3300.042.6040762
GTGGTAT3550.040.5755421
GGTATCA9250.039.6721271
ACCTATA350.001388291439.189812
GTATCAA18200.035.6150741
TATCAAC21150.030.4809652
GTCTTAT605.611427E-428.5799981
CTATAGC605.618485E-428.573864
CAACGCA24150.026.4086325
CCTATAG658.969825E-426.3758683
ATCAACG24550.026.2576873
AACGCAG24300.026.2456176
TCAACGC24950.025.6992934
AGAGTAC23850.025.30495311
CAGAGTA23900.025.25201210
ACGCAGA25550.024.9633757
GGAATAA1300.001367789224.53152570
TACACTC851.503925E-424.2037375
GCAGAGT26300.023.9907199
CGCAGAG26500.023.809668
Produced by FastQC (version 0.11.5)