FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830588
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA88030.4808837379027941No Hit
GTGTATTACTGTGCGAAGCCCGGTCCGGATTGTAGTAGTACCAGCTGCTATAATGACTACTGGGGCCAGGGGACC71350.3897654742629144No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG68820.37594477839907176No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59710.32617934783796243No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT53220.2907262584481052No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC52920.2890874407567405No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT52390.28619219616866276No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT48590.2654338387447093No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC46500.25401674216153497No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG44720.24429309052610418No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG43640.2383933468371911No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA43140.23566198401824986No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38100.2081298468033222No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT36750.20075516719218087No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35860.19589334137446549No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG35190.19223331519708423No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA33740.18431236302215462No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGA33370.18229115453613812No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA33300.18190876374148635No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGGT32570.17792097402583212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32500.17753858323118038No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32370.17682842889825565No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC31890.17420632059207206No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC31560.17240362113157084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31250.17071017618382728No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT30750.16797881336488604No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30190.16491968700767184No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT29290.16000323393357763No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29120.1590745705751376No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC28940.15809127996031874No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT28800.15732649837101523No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT27890.15235541804054217No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT27700.1513175001693445No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT27350.14940554619608562No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27210.14864076460678208No Hit
GTATTACTGTGCGAAGCCCGGTCCGGATTGTAGTAGTACCAGCTGCTATAATGACTACTGGGGCCAGGGGACC26510.14481685666026436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26150.14285027543062667No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA25990.14197623932856546No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT25190.1376060588182595No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA24820.13558485033224296No Hit
GTACTACTACAATCCGGACCGGGCTTCGCACAGTAATACACAGCCGTGTC24460.13361826910260527No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT24400.13329050556433233No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA24110.1317063151293464No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24020.131214669821937No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG24010.1311600425655582No Hit
GTCTCCAGCCACCGTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACTAG23950.13083227902728523No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACT23910.1306137700017699No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGGACACGTCG23850.13028600646349697No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23750.12973973389970872No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA23170.12657135302973688No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA23110.12624358949146394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23060.12597045320956982No Hit
GGCCAAAAGTGATCCAGTTATGATACTGCTGACAGTAATAAACTGCAAAA23020.12575194418405453No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG22650.12373073569803801No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC22640.12367610844165919No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21960.11996145500789909No Hit
CTCCCAGGGTCCTCATCTATCATGCATCCACCAGGGCCACTGGTATCCCA21740.11875965536756496No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21560.11777636475274612No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG21520.11755785572723082No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA21520.11755785572723082No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC21180.11570052901035076No Hit
GGATTGGGAGTGTCTATTATGGTGGGAGCACCTACTACAACCCGTCCCTC21130.11542739272845666No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21110.115318138215699No Hit
GTAGTAGTACCAGCTGCTATAATGACTACTGGGGCCAGGGGACCCTGGTC21040.11493574742104723No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG20780.11351543875519779No Hit
GTCTATTATGGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT20640.11275065716589423No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20570.11236826637124246No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC20420.1115488575255601No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20360.11122109398728713No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19980.1091452582448918No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19960.10903600373213417No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTA19760.10794345860455766No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA19720.10772494957904237No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC19410.1060315046312988No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCA19270.10526672304199525No Hit
ATCATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCA19120.10444731419631287No Hit
GTATCATAACTGGATCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT19100.10433805968355524No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT19030.10395566888890347No Hit
GTTATGATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT19030.10395566888890347No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT18820.10280849650494814No Hit
GTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC18440.1007326607625528No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT18410.10056877899341633No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC18390.10045952448065867No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC18370.10035026996790103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)