FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320734
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23980.18156570513063192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATATC200.007431741351.6219022
TCTAACA352.301982E-549.18047722
ATCTAAC306.379824E-445.90003621
TTAGTAA404.9404167E-543.2162436
GGTTAGT404.9659786E-543.17846734
GTATCAA48550.041.9765551
GTATAAG2050.040.3024751
TAATCTA459.7603246E-538.5023140
TATCAAC54000.037.72862
TATAAGA2100.037.692182
GGTATCA18750.037.454431
TAAGAGA2200.035.9748154
TAAACGT400.002462693534.8741150
CTTAATC400.002554395234.61512838
ATAAGAG2500.033.0342643
CAGAGTA61200.032.6139110
GTGGTAT7200.032.5125241
AGAGTAC62100.032.14124311
AACGCAG63800.032.0939066
TAAGAGG553.0247346E-431.76223653
Produced by FastQC (version 0.11.5)