FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307197
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76950.5886641416710717No Hit
CTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT72180.5521738498481866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56650.43337002762399246No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50390.38548130082917875No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48210.36880439597092096No Hit
CTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT48050.3675804029538011No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45870.35090349809554333No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTG44920.3436360395563943No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44730.3421825478485645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43810.3351445880001255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42690.3265766368802866No Hit
GGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT42490.3250466456088868No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG41770.3195386770318475No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTT38640.2955943136344407No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37850.2895508481124115No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT37450.2864908655696119No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG37430.28633786644247194No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA36260.27738741750478313No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAA35440.271114453292044No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA35420.27096145416490397No Hit
GTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT34070.26063401308295536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33490.25619703839589597No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32770.2506890698188567No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32630.24961807592887683No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31300.2394436339740682No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT30600.2340886645241689No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCC30350.23217617543491914No Hit
GGCCAAAAGTGTACAAGGGGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTG30250.23141117979921924No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA29090.22253723042510043No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAAAATCAGCAGAG27960.21389277974169155No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC27870.21320428366956168No Hit
CAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA27120.20746681640181242No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26590.203412339532603No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26370.2017293491340632No Hit
GCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC26170.2001993578626634No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25570.195609384048464No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT25180.19262590106923438No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24900.1904839132892747No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24550.18780642856432503No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24500.1874239307464751No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24110.1844404477672455No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC24080.1842109490765355No Hit
TTATTACTGCATGCACGCTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA23530.18000347308018608No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23080.17656099271953654No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22970.17571949752026667No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT22610.17296551323174703No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22090.16898753592610755No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGA21890.16745754465470775No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT21540.1647800599297581No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21470.16424456298476817No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21420.16386206516691823No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20900.15988408786127875No Hit
GATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG20670.15812459789916897No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGA19720.15085713936001996No Hit
GTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCC19630.15016864328789004No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19140.14642016467296054No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19080.1459611672915406No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18860.14427817689300082No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGC18630.14251868693089106No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18510.14160069216805118No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18360.14045319871450135No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC18100.1384642100616816No Hit
ATCCATTACTATGCAGGAGGCTCTGACTCGACCTGCAGGAGATGGAGGCC18040.13800521268026167No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG17990.13762271486241173No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGA17850.13655172097243184No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17320.1324972441032224No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17130.1310437523953926No Hit
CTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCT17130.1310437523953926No Hit
GTTTATTACTGCATGCACGCTCTACAAACCCCCTTGTACACTTTTGGCCA16910.1293607619968528No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16710.127830770725453No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16660.12744827290760305No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16570.12675977683547315No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16310.12477078818265341No Hit
CCTGCAGGTCGAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTG16270.12446478992837345No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTC16230.12415879167409351No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16210.12400579254695351No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16060.12285829909340366No Hit
ACCTACTACAACGCGTCCCTCAAGAGTCGAGTCACCATATCTGTAGACACGTCCAAGAACCAGTTCTCCCTGCAA15940.1219403043305638No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15850.12125180825843387No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15630.11956881785989411No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15620.11949231829632412No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15470.11834482484277427No Hit
GAGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCACGCTCTACAA15410.11788582746135434No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15330.11727383095279441No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15300.11704433226208445No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15130.11574383968139461No Hit
GGCACAGATTTCACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCACGCTCTA15090.11543784142711466No Hit
GGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT15000.11474934535498475No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14910.11406084928285484No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT14860.11367835146500488No Hit
ATGCAGGAGGCTCTGACTCGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA14820.11337235321072495No Hit
ATATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATC14770.112989855392875No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC14560.1113833645579052No Hit
GTGTACAAGGGGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATC14430.11038887023149532No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14370.1099298728500754No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT14330.10962387459579542No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13970.1068698903072758No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13920.10648739248942585No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAT13860.10602839510800591No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13800.10556939772658597No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13670.1045749034001761No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAA13360.10220341692950641No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13210.10105592347595659No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCA13180.1008264247852466No Hit
GATTTCACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCACGCTCTACAAACC13150.10059692609453662No Hit
AGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT13140.10052042653096664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3250.041.271361
TCGTATG856.366463E-1140.7129940
GTATCAA20200.039.1601141
TGGTATC3900.036.1454622
CGTATGC1102.7284841E-1134.62603841
TTAACGG709.679788E-734.5262635
GGTATCA8700.033.997481
TATCAAC23550.033.579292
TACACAT1305.456968E-1231.7386935
TAGGCAT1101.0822987E-931.3021229
CCGTTAA450.004706150430.55516
TGTTAGA11200.030.54866670
ATCAACG26050.030.2258763
AACGCAG25850.030.193716
ACTAGGC1151.7516868E-929.92395827
AGACTAG1151.7607817E-929.9090725
TCAACGC26300.029.8078254
CTCGTAT1052.2748281E-829.64633839
GTAGTTT1752.7906754E-729.51116670
CAACGCA26550.029.3976425
Produced by FastQC (version 0.11.5)