FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307197
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75500.5775717049534232No Hit
CTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT63790.4879907160129651No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51960.39749173230966717No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51830.3964972379832573No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45940.35143899504053333No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45100.3450130317006541No Hit
GGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT43640.3338440954194356No Hit
CTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT43000.32894812335095625No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42570.3256586421174467No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG42160.32252216001107714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41660.3186971818325776No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTG41100.31441320627265823No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG39140.2994192918129402No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT38330.293222827163771No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36100.2761634244876633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35580.27218544718202387No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA33990.26002201657439544No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33510.2563500375230359No Hit
GTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT33460.255967539705186No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33100.25321355541666635No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA32410.24793508553033705No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTT32100.24556359905966735No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAA31770.24303911346185772No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30130.2304931850363794No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT29970.22926919201925952No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29210.2234552251879403No Hit
GGCCAAAAGTGTACAAGGGGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTG27440.2099148024360521No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27280.20869080941893228No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAAAATCAGCAGAG26580.20333583996903298No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCC26160.2001228582990934No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25580.19568588361203398No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25470.1948443884127641No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25140.19231990281495442No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24700.1889539220178749No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24430.18688843380148518No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGA24420.18681193423791517No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA24340.18619993772935525No Hit
CAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA24150.18474644602152543No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23790.18199246173300582No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT23670.18107446697016594No Hit
GCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC23540.18007997264375608No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC23540.18007997264375608No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23390.17893247919020622No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23100.17671399184667652No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22990.17587249664740662No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT22710.17373050886744693No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22240.17013502937965738No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21550.1648565594933281No Hit
TTATTACTGCATGCACGCTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA21460.16416806342119816No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20990.16057258393340865No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20980.16049608436983867No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT20900.15988408786127875No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGA20270.1550646153563694No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19850.15185163368642982No Hit
GATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG19820.15162213499571986No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19680.15055114110574No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19420.14856215245292026No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19350.14802665550793034No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18620.1424421873673211No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18470.14129469391377122No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTC18360.14045319871450135No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18240.13953520395166144No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18170.13899970700667152No Hit
GGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT18040.13800521268026167No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17860.13662822053600185No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGA17800.1361692231545819No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17440.13341523886606227No Hit
CTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCT17240.13188524759466247No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17230.1318087480310925No Hit
GTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCC17190.13150274977681253No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16960.12974325981470275No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16920.1294372615604228No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT16690.12767777159831303No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG16640.1272952737804631No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16280.12454128949194346No Hit
GTTTATTACTGCATGCACGCTCTACAAACCCCCTTGTACACTTTTGGCCA16230.12415879167409351No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16160.12362329472910356No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC15980.12224630258484376No Hit
ATCCATTACTATGCAGGAGGCTCTGACTCGACCTGCAGGAGATGGAGGCC15910.12171080563985384No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGC15840.1211753086948639No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15530.11880382222419421No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT15100.11551434099068465No Hit
ATATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATC14500.11092436717648525No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14450.1105418693586353No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14340.10970037415936541No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14340.10970037415936541No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14280.10924137677794547No Hit
GTGTACAAGGGGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATC14180.10847638114224557No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13950.10671689118013582No Hit
CCTGCAGGTCGAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTG13810.10564589729015596No Hit
ACCTACTACAACGCGTCCCTCAAGAGTCGAGTCACCATATCTGTAGACACGTCCAAGAACCAGTTCTCCCTGCAA13680.1046514029637461No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13550.10365690863733622No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13530.10350390951019625No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13500.10327441081948627No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT13370.10227991649307641No Hit
GAGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCACGCTCTACAA13300.10174441954808647No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC13300.10174441954808647No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT13180.1008264247852466No Hit
GGCACAGATTTCACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCACGCTCTA13180.1008264247852466No Hit
AGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT13110.10029092784025669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA150.002398745968.6850118
CGATTGT400.00889802149.3203270
GTATCAA23800.043.139621
GTGGTAT3450.042.7987861
TATCAAC28050.036.7088782
TGTTAGA13300.036.09406770
TGGTATC4100.035.9970862
TATAAGA1750.035.303452
GGTATCA9100.035.0932581
AGAGCAT7900.034.9612470
AACGCAG29600.034.4453436
GTTAGCC400.002662859434.32283
ATAAGAG1800.034.32283
ATCAACG29950.034.15093
CAACGCA30200.033.758425
TCAACGC30350.033.4759064
GTATAAG2000.032.6216351
AGAGTAC31300.032.0285711
CAGAGTA31300.032.02734410
CGCAGAG31750.031.8977998
Produced by FastQC (version 0.11.5)