FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030783
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32200.15855953097893768No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21860.10764320954035957No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21630.10651064146193859No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21470.1057227680160805No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21100.1039008106725337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39800.039.1498371
TATCAAC46600.033.652622
GTGGTAT7100.032.943171
ATCAACG48050.032.637093
TCAACGC49000.031.8631744
CAACGCA49100.031.7998455
AACGCAG49950.031.1913936
AGAGTAC52150.029.28271711
CGCAGAG53450.029.1496418
CAGAGTA52750.029.1452510
ACGCAGA55500.028.0729437
GCAGAGT56050.027.7361099
GGTATCA23850.026.536541
ATTAGAG4050.024.6253783
AGTACGG48950.024.59163313
TATTAGA4500.024.455552
GAGTACG50350.024.04506312
GTATTAT1502.9833245E-822.931031
AATAGCG4400.022.3904862
CGTTTAT5900.021.32700355
Produced by FastQC (version 0.11.5)