FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518339
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11770.2270714725305254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA306.4418034E-445.804812
CCTATAC306.4418034E-445.804813
GGTATCA6150.040.7774541
GTGTAAT350.001375588139.261271
GTATCAA14300.038.197371
TATCAAC16300.033.0890582
GTGGTAT2000.032.6359251
AACGCAG17700.030.8629996
CAACGCA17650.030.5581685
ATCAACG17900.030.1313783
CAGAGTA18150.029.72207610
CTTAGGA703.9602233E-529.4459512
AGAGTAC18400.029.13150411
TCAACGC18500.028.9684474
TAAGTAG605.3386745E-428.82264138
CAAGCTA605.408857E-428.758433
ACGCAGA18900.028.7244327
CGCAGAG19250.028.0236748
GCAGAGT19800.027.4187749
GTCCTAA658.868125E-426.4258521
Produced by FastQC (version 0.11.5)